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基于GEO数据库探究RACGAP1表达与膀胱癌患者临床病理和预后的关系

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Clinical significance of RACGAP1 expression in bladder cancer: A bioinformatics analysis based on GEO database

摘要: 目的 探究Rac GTP酶活性蛋白1(RACGAP1)基因在膀胱癌组织中的表达情况及临床意义。 方法 从NCBI的基因表达汇编(GEO)数据库下载膀胱癌组织RACGAP1的表达数据和临床病理参数。分析RACGAP1基因在膀胱癌组织与正常组织中的表达差异,结合随访信息使用SPSS软件对RACGAP1表达与临床病理特征进行卡方检验,采用Kaplan-Meier法进行生存分析,并利用基因富集分析(GSEA)法分析受RACGAP1调控的相关基因。 结果 RACGAP1在正常膀胱组织中的表达水平为7.557±0.020,低于膀胱癌组织的7.790±0.028,差异有统计学意义(P<0.05)。膀胱癌组织中RACGAP1表达水平与年龄、侵袭性、T分期、N分期、疾病分级和复发有关(P<0.05)。RACGAP1低表达组和高表达组的5年总生存率分别为73.9%和56.6%(HR=0.47,95%CI:0.29-0.77,P<0.01),肿瘤5年特异生存率分别为91.6%和70.9%(HR=0.33,95%CI:0.17-0.67,P<0.01)。RACGAP1高表达样本富集了MYC信号通路、精子发生、未折叠蛋白反应、G2M检查点、E2F转录因子、MTORC1信号、有丝分裂纺锤体、PI3K/AKT/mTOR途径和DNA修复修复相关的基因集。 结论 RACGAP1在膀胱癌组织中高表达,与膀胱癌患者的临床病理及预后显著相关,可作为反映膀胱癌患者预后的生物学标志物和潜在的膀胱癌防治靶点。
Abstract: Objective To explore the expression of Rac GTPase activating protein 1 (RACGAP1) gene in the bladder cancer tissues in the Gene Expression Omnibus (GEO) database and its impact on the clinicopathological characteristics and prognosis of bladder cancer. Methods The expression profile of RACGAP1 and its clinical parameters of bladder cancer tissues were downloaded from GEO database. The relationship between the RACGAP1 expression and the clinicopathological characteristics of bladder cancer were analyzed. Combined with follow-up information. SPSS software was used to test the expression and clinicopathological features of RACGAP1.Gene set enrichment analysis (GSEA) were used to explore the regulatory gene sets of RACGAP1. 、 Results The expression level of RACGAP1 in normal bladder tissues was 7.557±0.020, lower than 7.790±0.028 of bladder cancer tissues, and the difference was statistically significant (P<0.05). BC patients in RACGAP1 low expression group were associated with better invasiveness ,T staging , N staging ,classification, and a trend of better recurrence than those in RACGAP1 high expression group (P<0.05). Bladder cancer patients in RACGAP1 low expression group were associated with better cancer specific survival (HR=0.47, 95%CI: 0.29-0.77, P<0.01) and overall survival (HR=0.33, 95%CI: 0.17-0.67, P<0.01) compared with those in RACGAP1 high expression group. The results of GSEA suggested that BC samples in RACGAP1 high expression group were enriched in “MYC targets V1”, “Spermatogenesis”, “Unfolded protein response”, “G2M checkpoint”, “E2F targets”, “Mtorc1 signaling”, and “Mitotic spindle”. Conclusions RACGAP1 was highly expressed in bladder cancer and significantly associated with clinicopathological parameters and prognosis of bladder cancer patients. RACGAP1 could be a potential target in the diagnosis and treatment of bladder cancer." " "

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[V2] 2024-01-03 21:03:50 ChinaXiv:202003.00054V2 下载全文
[V1] 2020-03-16 17:17:04 ChinaXiv:202003.00054v1 查看此版本 下载全文
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