Subjects: Biology >> Bioengineering submitted time 2017-11-26 Cooperative journals: 《中国生物工程杂志》
Abstract：抗坏血酸在植物的生长发育和抗逆过程中具有重要作用。主要概述了抗坏血酸在模式植物和园艺植物中的代谢途径，并且从转录水平、翻译水平和遗传转化等几个方面综述了多个不同基因对抗坏血酸
Subjects: Biology >> Bioengineering submitted time 2017-11-26 Cooperative journals: 《中国生物工程杂志》
Abstract：抗坏血酸在植物的生长发育和抗逆过程中具有重要作用。主要概述了抗坏血酸在模式植物和园艺植物中的代谢途径，并且从转录水平、翻译水平和遗传转化等几个方面综述了多个不同基因对抗坏血酸
Subjects: Biology >> Bioengineering submitted time 2017-11-21 Cooperative journals: 《中国生物工程杂志》
Abstract：抗坏血酸在植物的生长发育和抗逆过程中具有重要作用。主要概述了抗坏血酸在模式植物和园艺植物中的代谢途径，并且从转录水平、翻译水平和遗传转化等几个方面综述了多个不同基因对抗坏血酸水平的调控机制。以期为深入研究抗坏血酸在植物（尤其是园艺植物）中的代谢调控机制提供参考。
Subjects: Biology >> Bioengineering submitted time 2017-11-21 Cooperative journals: 《中国生物工程杂志》
Abstract：抗坏血酸在植物的生长发育和抗逆过程中具有重要作用。主要概述了抗坏血酸在模式植物和园艺植物中的代谢途径，并且从转录水平、翻译水平和遗传转化等几个方面综述了多个不同基因对抗坏血酸水平的调控机制。以期为深入研究抗坏血酸在植物（尤其是园艺植物）中的代谢调控机制提供参考。
Subjects: Biology >> Bioengineering submitted time 2017-06-01 Cooperative journals: 《中国生物工程杂志》
Abstract： Abstract In order to understand stress-inducible expression characteristics of sorbitol-6-phosphate dehydrogenase (S6PDH) promoter (S6PDHp) from apple, a series of 5’ deletions of S6PDH promoter were fused with GUS reporter gene by Gateway and introduced into arabidopsis by agrobacterium-mediated transformation. The expression characteristics of S6PDHp were validated by GUS activity changed in transgenetic arabidopsis against low temperature and exogenous ABA. Molecular detection analysis revealed that 4 5’ deletions of S6PDHp were fused with GUS reporter gene successfully (i.e. pGWB433-S6PDHp1, pGWB433-S6PDHp2, pGWB433-S6PDHp3 and pGWB433-S6PDHp4) and relevant transgenetic arabidopsis were obtain in this study. In low temperature treatment, GUS activity in pGWB433-S6PDHp3 transgenetic line increased markedly, while had obvious change in others deletions transgenetic lines. In exogenous ABA treatment, GUS activity in others deletions transgenetic lines increased dramatically except pGWB433-S6PDHp4. These results suggest that S6PDHp can be induced by low temperature or exogenous ABA, however, there were different response in every deletions. These indicated that positive and negative cis-regulatory elements in response to abiotic stress may co-existed in the promoter region (-2396bp to -236bp) of S6PDH.
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